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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in several tumors, which leads to chromosome instability, and thus may contribute to tumorigenesis. This protein was discovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential position in endorsing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already described. Two further splice variants have been reported but in depth nucleotide sequence info is not yet readily available.

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Blocking peptides are peptides that bind precisely into the focus on antibody and block antibody binding. These peptide ordinarily contains the epitope recognized from the antibody.

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This guidebook outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...

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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...

Antibodies certain to the blocking peptide not bind towards the epitope around the goal protein. By evaluating the staining from the blocked antibody as opposed to the antibody by yourself, you can see which staining is specific.

Focus on Facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose members are characterised by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...

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